Coding

Part:BBa_K1371003

Designed by: Yiran Wu   Group: iGEM14_SCUT-China   (2014-10-03)

DH-ER


DH Part

The function of DH domain is dehydrating the extended unit and enabling it to form the α, β- enol ester bond. In DH domain catalyzed reactions, the oxhydryls in the extended units and the hydrogen atoms binding to the neighbor carbon atoms are removed by DH domains together and form. After catalysis, α, β-enol ester bonds are formed in the polyketide chains.


ER Part

The function of ER domain is deoxidizing the extended unit and enabling it to form the saturated methylene. The mechanism of ER domain catalyzed reactions is similar to KR domain. In ER domain catalyzed reactions, NADPHs provide hydride ions and ER domains provide protons to the complexes and deoxidize carbon-carbon double bond to form the saturated methylene.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 507
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 352
    Illegal NgoMIV site found at 907
    Illegal NgoMIV site found at 1431
    Illegal NgoMIV site found at 1528
    Illegal NgoMIV site found at 1615
    Illegal NgoMIV site found at 1720
    Illegal NgoMIV site found at 1936
    Illegal AgeI site found at 1081
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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